(oops, had to resend, forgot to change the destination to <distutils-sig@python.org>) biopython-1.77, for instance, when installed into a virtualenv with pip3, has many of these shebangs: #!/usr/bin/env python And they are all over the place. They are: ./site-packages/Bio/bgzf.py: ./site-packages/Bio/PDB/parse_pdb_header.py: ./site-packages/Bio/PDB/PDBList.py: ./site-packages/Bio/Restriction/__init__.py: ./site-packages/Bio/Restriction/Restriction.py: ./site-packages/Bio/Restriction/PrintFormat.py: ./site-packages/Bio/Restriction/Restriction_Dictionary.py: ./site-packages/Bio/Wise/__init__.py: ./site-packages/Bio/Wise/psw.py: ./site-packages/Bio/Wise/dnal.py: ./site-packages/Bio/UniProt/GOA.py: ./site-packages/Bio/SeqUtils/__init__.py: Regards, David Mathog