[Kwant] Is the transmission/conductance matrix at zero temperature calculated at E = 0?

Hi All, I have a general question about kwant package. In the kwant paper (https://downloads.kwant-project.org/doc/kwant-paper.pdf), I presume the conductance matrix given in Eq. (10) is for zero temperature calculation. I also presume that the conductance matrix G should be dependent on energy E, i.e., G(E). I am wondering if the output from the transmission matrix t(E) (used to calculate G(E)) of kwant is calculated at E = 0, i.e., what we have is actually t(E=0) and G(E=0). Although this looks like that the Fermi level is fixed at E = 0, the actual Fermi level (e.g., relative to charge neutral point) can be changed via the onsite potential. Please correct me if I have the wrong understanding. Thanks! -- Jiuning Hu