Assuming you mean manual 3D segmentation, I second Greg’s recommendation of ITK-SNAP for fully manual segmentation. For semi-automated work, I recommend Ilastik (ilastik.org).

On 5 Apr 2017, 7:43 PM -0400, Gregory Lee <grlee77@gmail.com>, wrote:

I was wondering if anyone on the list knew about software to do 3d segmentation.

Thanks

Vighnesh

A nice, free GUI-based tool for interactive 3D segmentation is itk-snap
http://www.itksnap.org/

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