Dear all, I meet some problem to open/display 12bits tif (16 bits) gray images. I can open an image but I fail to display it: import skimage path='/media/Elements__/Science/Biologie/QTeloFISH/myoblastes HPS/JPP55_nFF/16/DAPI' image='/1.tif' from skimage import io as io print skimage.io.plugin_info('matplotlib') #skimage.io.use_plugin('matplotlib', 'read') #dapi=io.imread(path+image,plugin='matplotlib') dapi=io.imread(path+image) print type(dapi) print type(dapi) print 'dapi.dtype',dapi.dtype io.imshow(dapi) #import pylab #pylab.imshow(dapi) #pylab.show() Then I get the following error: *<type 'numpy.ndarray'> <type 'numpy.ndarray'> dapi.dtype object Traceback (most recent call last): File "/home/claire/.spyder2/.temp.py", line 28, in <module> io.imshow(dapi) File "/usr/local/lib/python2.6/dist-packages/skimage-0.4-py2.6-linux-i686.egg/skimage/io/_io.py", line 149, in imshow return call_plugin('imshow', arr, plugin=plugin, **plugin_args) File "/usr/local/lib/python2.6/dist-packages/skimage-0.4-py2.6-linux-i686.egg/skimage/io/_plugins/plugin.py", line 88, in call return func(*args, **kwargs) File "/usr/local/lib/python2.6/dist-packages/skimage-0.4-py2.6-linux-i686.egg/skimage/io/_plugins/pil_plugin.py", line 108, in imshow Image.fromarray(arr).show() File "/usr/lib/python2.6/dist-packages/PIL/Image.py", line 1886, in fromarray raise TypeError("Cannot handle this data type") TypeError: Cannot handle this data type * The default plugin is PIL.If I try to switch to matplotlib (or freeimage), the plugin doesn't seem available: *Traceback (most recent call last): File "/home/claire/.spyder2/.temp.py", line 16, in <module> dapi=io.imread(path+image,plugin='matplotlib') File "/usr/local/lib/python2.6/dist-packages/skimage-0.4-py2.6-linux-i686.egg/skimage/io/_io.py", line 72, in imread img = call_plugin('imread', fname, plugin=plugin, **plugin_args) File "/usr/local/lib/python2.6/dist-packages/skimage-0.4-py2.6-linux-i686.egg/skimage/io/_plugins/plugin.py", line 86, in call (plugin, kind)) RuntimeError: Could not find the plugin "matplotlib" for imread.* I can open the same image with plugin='pil' but I fail to display it: *uint16 (1024, 1536) Traceback (most recent call last): File "/home/claire/.spyder2/.temp.py", line 28, in <module> io.imshow(dapi0) File "/usr/local/lib/python2.6/dist-packages/skimage-0.4-py2.6-linux-i686.egg/skimage/io/_io.py", line 149, in imshow return call_plugin('imshow', arr, plugin=plugin, **plugin_args) File "/usr/local/lib/python2.6/dist-packages/skimage-0.4-py2.6-linux-i686.egg/skimage/io/_plugins/plugin.py", line 88, in call return func(*args, **kwargs) File "/usr/local/lib/python2.6/dist-packages/skimage-0.4-py2.6-linux-i686.egg/skimage/io/_plugins/pil_plugin.py", line 108, in imshow Image.fromarray(arr).show() File "/usr/lib/python2.6/dist-packages/PIL/Image.py", line 1886, in fromarray raise TypeError("Cannot handle this data type") TypeError: Cannot handle this data type* Does io.imshow() support only 8bits image? I can open and display the image with readmagick/pylab. Best regards Jean-Patrick