Yes, that's what I mean. I haven't used it so I can't offer guidance there but you might want to fiddle with the parameters / update your scikit-image to the github master version. Regarding your source images, it looks like there is a nice signal separating touching cells. You might want to run an edge detector (e.g. Ilastik) or even just morphologically opening your images prior to thresholding. Juan. On Fri, Mar 27, 2015 at 7:45 AM, Claiborne Morton <claiborne.morton@gmail.com> wrote:

Also Juan, When you say, "There have been some recent developments on that function," are you referring to the peak_local_max() function? On Thursday, March 19, 2015 at 6:07:58 PM UTC-4, Claiborne Morton wrote:

...

Hey guys, Im still having trouble finding ways to separate touching particles if the are not both circular. Further when dealing with elliptical shapes, a single particle tends to incorrectly get cut in half. Any ideas how I could change parameters in the water-shedding function to correct for this? Attached are a few problem cases so you can see examples.

Thanks, Clay [image: Inline image 1]

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