I went ahead and set pip_pre=true in pytest-twisted's tox.ini to try it out and got warnings/errors with PyPy 3.7 about use of t.i.d.returnValue claiming it should only be used in @inlineCallbacks decorated functions even though the location complained about is such. Only PyPy 3.7, not PyPy 3.6 nor CPython 3.7. Linux, macOS, and Windows all failed.
Ticketed as https://twistedmatrix.com/trac/ticket/10093 .