I've yet to hear back from anyone testing the prerelease - if you're able to, please do!
Tested with Autobahn (WebSocket) + Twisted Web based stuff .. no issues found.
It also supports SSL chain certificates, which should also fix up some issues using TLS with Twisted - this would also be great for people to test, and will hopefully remove a bunch of monkeypatching in everyone's code.
Still using monkey patching (for various other SSL things), but I can confirm "no issues"
See bottom of that page: "HTTP server signature TwistedWeb/13.2.0pre1"
Thanks for the hard work!!