little question speed encondings

[Linos]

I know that this can be evident for most of the people but i would like to know
the reply and the reasoning if possible:

Should my python application have a difference in speed of execution after
change the encoding header of the file from nothing (ascii) to latin-1 or utf-8?
If yes, only when the .pyc files are created or even with .pyc or .pyo files?

Regards,
Miguel Angel.