Advice on improving a analysis on images of nanoparticles

[Adam Hughes]

Thanks for the help Stefan.  Here is a direct link that anyone can view:  

http://www.scribd.com/doc/185189653/Scikit-Test-Notable

To download without an account, I am not familiar with any hosting 
solution, but if you guys have any recommendations I'd love to hear them.

Thank you for you help, I will test out the methods you suggested

On Sunday, November 17, 2013 4:27:12 AM UTC-5, Stefan van der Walt wrote:
>
> Hi Adam 
>
> On Fri, Nov 15, 2013 at 7:02 PM, Adam Hughes <hughes... at gmail.com<javascript:>> 
> wrote: 
> > The images are in a .pdf can be downloaded directly here. (Just a hair 
> too 
> > big to attach) 
>
> This download requires a login--could you please provide us with a 
> direct link / HTML page showing the images? 
>
> > LOWCONTRAST - Can the contrast in these images be enhanced automatically 
> in 
> > SKI? 
>
> We have several histogram equalization algorithms, including Contrast 
> Limited Histogram Equalization, which should do the trick. 
>
> > NONCIRC - Particles appear non-circular due to stigmation offset in 
> > microscope.  Is it possible to reshape them/make them more circular? 
>
> It depends on what you mean by "make them more circular".  We have 
> geometric transformation utilities in `skimage.transform`. 
>
> > WARPED - Images that have artifacts, or uneven contrast, due to 
> aberrations 
> > in SEM beam during imaging.  I'm especially interested in removing 
> uneven 
> > contrast. 
>
> Localized contrast equalization in the `skimage.filter.rank` module might 
> help. 
>
> > WATERSHED - These images have overlapping AuNPs, and I had hoped that 
> SKI's 
> > watershedding routines might help disentangle them.  The watershed 
> > segmentation guide indicates that there are several ways to approach 
> this 
> > problem. 
>
> See Juan's post here: 
> https://groups.google.com/d/msg/scikit-image/4z-hPiFFDj8/2gIDMxfGrU4J 
>
> Regards 
> Stéfan 
>
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