I wrote: >>> nwalign.global_align("PLEASANTLY", "MEANLY", gap_open=-5, gap_extend=-5, matrix='./BLOSUM62.txt') >>> ('PLEASANTLY', '-MEA--N-LY') I forgot to mention that I got my BLOSUM62.txt from http://www.ncbi.nlm.nih.gov/Class/FieldGuide/BLOSUM62.txt in case that matters. Laura