[Image-SIG] manipulating image for counting microbial cells

David Berthelot d_berthelot at yahoo.com
Thu Sep 24 21:47:13 CEST 2009

I do some pretty fancy image manipulation and I use numpy + PIL to do my processing. What you are describing can easily be achieved by using those packages together.

From: Chuck Pepe-Ranney <cpepera at mines.edu>
To: image-sig at python.org
Sent: Monday, September 21, 2009 2:28:04 PM
Subject: [Image-SIG] manipulating image for counting microbial cells

I am wondering if I can use PIL to manipulate some fluorescent microscopy images.  Specifically, I will have two images of the same field but each will be taken under different emission filters.  What I need to do is loop through the pixels in the first image multiply each pixel's intensity by a factor then divide it by the intensity of the spatially corresponding pixel in the second image.  There are more steps to follow but they are seemingly routine image manipulation tasks.  The images will be in tiff format.  Would PIL be a suitable solution to this problem?
Thanks for the help in advance,
- Chuck

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